— In this study, a novel, rapid, economical, accurate, reproducible and sensitive analytical method for the determination of Nitenpyram using reverse-phase high-performance liquid chromatography with UV-Visible detector has been reported. This method is equally useful and valid for the detection and quantification of Nitenpyram in different dosage formulations and raw material forms with outstanding recoveries up to 100%. The method has been validated with superb linearity value R² = 0.9999. LOD was measured as 0.51 mg/L while the LOQ was 1.69 mg/L. The analytical method has shown high precision (RSD @ 0.17%) while the accuracy measured in terms of recovery percentage was approximately 100% under optimized experimental conditions. Finally, the robustness results of the proposed method by altering the flow rate and the mobile phase concentration ratios were within the permissible accepted values in terms of Relative Standard Deviation (RSD ≤ 2%). So, the proposed method can be used in quality control laboratories of the pesticide industry at a commercial scale even for the determination of Nitenpyram in samples such as Soluble Liquid (SL), Wettable Powder (WP), Water Dispersible Granules (WDG), Emulsifiable Concentrate (EC) and Technical (Pure Active Ingredient), etc.

Keywords:  HPLC-UV, Nitenpyram, Insecticide, Method Validation