The cross-sectional study is done on healthy volunteer blood donors from Lady Reading Hospital (LRH), Al-Khidmat Hospital (AKH), and Blood Donation Center. A total of 300 individuals of male different ages were included in the study in which most of them 23-28 years old. The aim of the study is to find variations O blood group and Coagulation Profiles in healthy blood donors. To better understand the post bleeding complication in blood donors. The most common blood group were B+ (34%, n=106), A+(21%, n=65), O+(20%, n=60).  The minimum value for prothrombin time (PT) is 14S and the maximum value is 40S with mean and standard deviation of 17.11±5.54S, activated partial thromboplastin time (APPT) minimum value is 25S and the maximum is 44S with mean and standard deviation is 35.54±2.39. Correlation shows that blood groups, AB- had the lowest APPT (34.00S) and PT (14.75 S), while O- had the highest APPT (36.75S) and PT (18.75 S) after donation. The other blood groups, including AB+, B+, A-, O+, A+, and B-, had intermediate values for both APPT and PT. On the basis of blood group there is no fluctuation record in coagulation profile.