Objective

For improved understanding of stress mechanism and machinery, analysis of regulatory pathways of multiple transcripts (i.e., non-coding RNAs) and salt stress-responsive genes is a vital need.

Methodology

In the current study, seeds of two cultivars of Brassica napus Dunkled and Cycloned were used in completely randomized design with three replicates. Plants were categorized into two S1 and So treatments of NaCl. Growth parameters of plants were measured, and effects of salinity were observed. Transcriptome analysis was carried out after isolation of total RNA by analyzing protein coding 10 upregulated and 10 downregulated genes on NCBI, KEGG, UniProt, Interpro. Phylogenetic analysis of genes was done by using different analytical software i.e., NCBI BLAST, MUSCLE for alignment, BioEdit analytical tool for editing the sequences and MEGA7 was used for phylogenetic tree to describe the ancestral relationship of plant genes with other identified genes.

Results

Results showed that the CRM-domain protein was involved in the mitochondrial splicing introns, which was ultimately very crucial for growth and stress responses of plants; and, for all stress reducing mitochondrial functions.

Conclusion

According to the results, functions of selected upregulated and downregulated genes of Brassica napus were predicted.

Keywords: Canola, non-coding RNAs, Brassica napus, salt stress.