Osteoporosis is the disease in which there is low bone density which leads to the low mechanical strength and the chances of the bone fracture increases. It usually begins to appear as acute back pain due to pathological vertebral compression or episodic groin or thigh pain due to hip fracture. This disease is becoming highly prevalent worldwide. It can be due to multiple causes either primary or secondary cause. Current study was carried to find the association of osteoporosis with physiological and biochemical variables of medical importance. The data was collected by non-sampling technique from the local clinical setup. 50 sample size was taken which included both genders of 18-60 age. By their consent, alkaline phosphatase, calcium, BMI, sono bone graph were measured. Bone graph was taken by SONO bone gadget. Other tests were done by taking their blood sample in lab. The result has shown that underweight and obese have more chances of osteoporosis. Increased calcium levels in body would increase the t-score leading to osteopenia. Alkaline phosphatase was increased among osteoporosis patients. Study concluded that there is association of alkaline phosphatase and calcium with osteoporosis. Still investigation is needed to find the association of secondary causes with osteoporosis.