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EVALUATION OF IN VITRO ANTI-ARTHRITIC AND ANTIOXIDANT ACTIVITIES OF EXTRACTS OF COTULA ANTHEMOIDES L.
Cotula anthemoides L. commonly recognized as peeli booti (babuna), is ubiquitous globally particularly in warm regions of the world. This plant has been practiced in rural areas for pain management associated with arthritis. Some of its constituents discovered in previous studies have been speculated to possess antioxidant properties as well.
Aim: Current study manifests the in vitro anti-arthritic and antioxidant activities on the plant. Proximate analysis, phytochemical analysis (primary and secondary metabolites) UV visible scanning and FTIR were performed on the powder as well as on the extracts of plant.
Results: The results came out to be as moisture content 5%, total ash 15.23%, acid insoluble ash 0.99%, water soluble ash 9.66%, sulphated ash 16.69%, alcohol soluble extractives 10.25%, and water-soluble extractive values 62.95% all the values came out to be under range of British Pharmacopeia. Different extracts of plant of C. anthemoides L. were squeezed out by hot extraction technique. UV visible scanning and FTIR scans demonstrated the
characteristic features and presence of various functional groups. The antioxidant activity was investigated following DPPH, phosphomolybdenum and ABTS models using ascorbic acid as standard while anti-arthritic activity was analyzed by inhibition of protein denaturation method using standards diclofenac sodium and acetyl salicylic acid.
Conclusion: Study results revealed that plant possesses both activities and has the potential to be explored further for its ultimate use in future medications.
Keywords: Cotula anthemoides L., DPPH, anti-arthritic,antioxidant, phosphomolybdenum, ABTS
