The current study was aimed to determine the antibacterial potential of Bidens bipinnata L. against bacterial strains isolated from cattle waste. The isolated bacteria included Bovine brucellosis, Bovine abortus, Bacillus anthracis, Campylobacter coli, Mycoplasma           bovis, Campylobacter jejuni and Mycoplasma synoviae.  Mycoplasma synoviae represented high resistance to Cephalosporins (Cefoxitin 100%, Cefuroxime 100%, Cefoperazone 100%). Campylobacter jejuni and Bovine brucellosis showed 100 % resistant to Ampicillin and Cefoxitin, 90 % to Cefuroxime and Cefoperazone, while there was 75 % resistance to Cefotetan. Campylobacter jejuni and Bovine brucellosis were 65 and 70 % resistannt to Erythromycin. The MAR index for Campylobacter jejuni and Bovine brucellosis was 0.55 while for Mycoplasma synoviae, it was 0.44. Different concentrations of the aqueous extract of the plant were implanted against the resistant bacteria and there was potent antibacterial activity of the plant extract against tested bacteria. It can be concluded that Bidens bipinnata has the potential to inhibit/kill the multi-drug resistant bacteria. Active components of this plant may be isolated and should be used against various bacteria to further confirm the antibacterial potency of the plant.

Key words: Bacterial strain, Cattle waste, Multi-drug resistant bacteria, drugs.