Since the beginning of human civilization, medicinal plants are utilized for health benefits. However, due to modernization in science and technology, such plants are re-evaluated for their proficiency to treat diseases from different aspects. The dried fruit peel is used as an asthmatic, and cough suppressant and in throat problems by the local community. In the same way, the aqueous peel extract (APE) of Punica granatum L. was used to screen for Alpha-glucosidase, and pharmacological and biological activities, via; in vivo and in vitro approaches. The antipyretic effect of the aqueous peel extract (APE) of Punica granatum was taken under consideration by using albino mice via; in vivo. Similarly, the APE of Punica granatum L. was given orally at concentrations of 50, 100 and 150mg/kg to the mice model to check the fever reduction potential, induced by brewer’s yeast, and paracetamol was used as standard. The body temperature of mice was measured rectally using a digital thermometer. All the concentrations were found effective but 150mg/kg was very good with results of 38.8±.15a after 18 hours of fever induce by brewer’s yeast. The APE of Punica granatum possesses dose-dependent alpha-glucosidase activity with 500 concentration showed results of 69.1±3.4 with IC50 of 410.37. Additionally, the selected bacterial and fungal strains were positively inhibited by APE with 2mg/mL concentration. It was concluded that the Punica granatum has hidden chemical constituents, which are to be isolated to treat simple to chronic diseases.

Key words: phytochemicals, antipyretic, medicinal plant, pomegranate, pharmacological activities.