Systemic lupus erythematosus is an autoimmune systemic disease of the connective tissue, characterized by the formation of many antibodies to its own cells and their components with the development of immune inflammation with damage to many organs and systems. Purpose of the research. To assess the prognostic significance of biochemical markers of cytokines for the development of kidney damage in systemic lupus erythematosus. Materials and methods. Genetic research methods included: isolation of genomic DNA. For this, venous blood was assessed in a volume of 5 ml and taken into sterile tubes containing EDTA (ethylenediaminetetraacetic acid). Isolation of genomic DNA was carried out by the sorbent method using the reagents of the innuPREP DNA Micro Kit. The DNA concentration was measured using an Eppendorf photometer (Germany). The quality of the obtained DNA samples was assessed on the basis of the optical density of the DNA solution in the region of the protein and nucleic absorption spectra (280 and 260 nm). Statistical data processing was carried out using the Statistica 10 statistical software package. Results. The frequency of occurrence of the minor T allele among patients with SLE did not practically differ from that in the control group (p = 0.08). The frequencies of the CC, ST and TT genotypes between the studied groups also did not differ (p = 0.05, p = 0.03 and p = 0.001, respectively). Further, the frequency of alleles and genotypes of the C / T polymorphism (-511) of the IL-1β gene was assessed depending on the duration of the disease. Taking into account the distribution of the duration of the course of SLE by percentiles, three groups of patients with the duration of the disease from 0 to 9 years (36.4%, 16 people), from 10 to 21 years (39.4%, 18 people) and from 22 to 50 years (24.2%, 11 people). There were no statistically significant differences in the distribution of genotypes among all patients with SLE (p = 0.204, Pearson χ2 = 5.93). The frequency of occurrence of the minor T allele and the TT genotype did not significantly differ in the groups with SLE duration of 0-9 years, 10-21 years, and 22-50 years. The minor TT genotype was less often detected in patients with SLE duration of 10-21 years, in contrast to patients with SLE duration of 0-9 years (p = 0.078), however, the differences found did not reach statistical significance. Conclusion: Thus, when studying the polymorphism (-1082) G / A of the IL-10 gene, it was shown that in patients with SLE, the GG genotype was significantly less common, and the minor allele (-1082) A was significantly more frequent, in contrast to the control group.

Key words: gene polymorphism; alleles; systemic lupus erythematosus; inflammatory nephritis; Toll-like receptors; interleukins; genotype.