Xenobiotics are foreign substances that are not part of the cycle. Many synthetic surfactants entering the body of mammals, including humans, cause a number of allergic reactions, and also cause infectious diseases, acting as contaminants acting on the body through food. Purpose of the research. Conduct a study of the metabolism of xenobiotics and drug-drug interactions under experimental conditions. Methods and materials: To achieve this goal, the results of these 20 experimental animals (mice of the Vistar genus, weight - 14-18 g, both sexes) were analyzed. As a material for the study, a biopsy of liver tissue was performed after exposure to drugs (doxirubicin) on days 3, 5, 10 of the experiment. Hepatocytes were stained with hematoxylin-eosin, standard wiring was used. We used iron (II) sulfate, potassium ferrocyanide, acetic acid, potassium phosphate, hydrochloric acid, sodium chloride, sodium hydroxide. Statistical processing was performed using the Student-Fisher test, the nonparametric Mann-Winney test, and the Kraskes-Wallis test. Results. The effect of ferrocyanide oxidation on the substrate activity of hepatocytes showed that at the beginning of the experiment (0-5 min) the content of this substance was at the level of 9.5 ± 0.05 μmol / L, while the content of HIF and peptide (4.1 ± 0.05 and 6.3 ± 0.05, p≤0.05, μmol / L). Within 30 minutes, the content of ferrocyanide increased to 12.0 ± 0.05 (p≤0.05), while the content of HIF and peptide did not increase (p≤0.05). When evaluating the effect of iron (II) sulfate on the metabolic activity of macrophages of hepatocytes, it was found that the state of these cell organelles when exposed to iron (II) sulfate without an enzyme at 0-5 minutes and 25-30 minutes was the same and amounted to 4.5 ± 0.05 ( p≤0.05) μmol / L, while in the state without an inhibitor in a time interval of 5-10 minutes it reached values of 4.5 ± 0.05 (p≤0.05) μmol / L, and at 15-20 minutes reached values of 1.8 ± 0.05 (p≤0.05) μmol / l and was the minimum value for the entire period of the influence of iron (II) sulfate. Conclusion: Summarizing the above, we can conclude that dcosirubicin has an ambiguous isoforms of cytochrome P450, since different classes of this monooxygenase react differently with this drug.

Key words: hypoxia-inducible factor; xenobiotics; doxirubicin; experiment; mouse