Low serum vitamin D3(Vit D3) and calcium levels as well as high parathyroid hormone (PTH) levels are the most important risk factors in osteoporosis especially after menopause.This study was performed to observethe differences in serum calcium(mg/ml), vitamin D3 (ng/ml) and parathyroid hormone (PTH) (pg/ml)level in obese post-menopausal women as compared to normal weight (control) post-menopausal women. This was a cross sectional study. Data were collected through a questionnaire from different localities of Karachi city. We selected 13 control (18.5-22.9 Kg/m²) and 71 obese (BMI>25Kg/m²)females of age group 40-55 yearswith ceased menstruation for at least one year. BMI of subjects was calculated by using their weight (Kg) and height (m) and Asian BMI cut-offs were use to categorized the females.Blood samples collected from respective subjects, after sampling serum and plasma was separated. Levels of Vitamin D3 and PTH hormone were estimated with the help of ELISA Kit, while serum Calcium was through colorimetric method. Serum calcium and Vit D3 levels of obese menopausal women were significantly reduced. However, PTH levels of obese postmenopausal women were found significantly elevated than controlfemales that indicate greater risk of bone turn over after menopause especially in obese women.

Keywords: Menopause, Obesity, Serum calcium, Vit D3, PTH