Background and Objective: Selected medicinal plants have been investigated as an alternative source for the treatment of Streptozotocin (Stz) induced diabetes. This aim of current study was to assess the anti-diabetic effects of selected plants formulation, Tribulus terrestris, Prunus dulcis, Cicer arietinum and Azadirachta indica in diabetic rats induced by streptozotocin.  Plants included in this phytoformulation have shown anti-oxidant, anti-diabetic, and anti-inflammatory properties evident in previous researches.

Material and Methods: Male albino rats were given a single dose of Stz (45 mg/kg) interperitoneally to induce diabetes followed by checking of blood sugar levels, feed and water intake, body weight and various biochemical parameters.

Treatment: Aqueous (4ml/kg) and ethanolic (2ml/kg) extracts of phytoformulation were prepared and for 21 days given orally to the diabetic rat groups.

Results: The treatment showed reduced glucose levels in random and fasting blood glucose tests, increased body weight, considerably decreased levels of ALT, AST, ALP, Hb1AC, serum urea, creatinine, total lipids, cholesterol, triglycerides, LDL and VLDL, whereas significant elevated levels of HDL. Histological analysis of pancreas also indicate that the extract improved the Islets of Langerhans and increased the number of β cells.

Conclusion: The current study has shown that the novel plant composition used has significant anti-diabetic effects and improved various biochemical parameters in rats. It is also suggesting that this plant composition is an easily accessible and cheap alternative medicine for diabetes with least side effects.

Keywords: Diabetes Mellitus, Streptozotocin, Phytoformulation, Tribulus terrestris, Prunus dulcis, Cicer arietinum and Azadirachta indica, islets of Langerhans