Malaria is a mosquito borne disease caused by plasmodium parasite and transmitted by the bite of infected mosquitoes. Malaria is life-threatening disease in Assam. Only female mosquitoes spread the malaria parasites. Malaria also keeps children from going to school, families from investing in their future and communities from prospering. In 2018 an estimated 228 million cases of malaria occurred worldwide. Most area in Assam are considered high risk for malaria because of its physiographic condition. Malaria infects all month of a year in Assam but it varies from time to time and place to place. Controlling malaria in the forest areas of Assam has remain a challenge from past. Various factors like topographical, environmental and climatic factors control the number of malaria infected case in the study area. Temperature has influential role for malaria transmission in the study area. This study will explain the correlation between malaria cases and temperature in Assam. The main objective of this study is to investigate the number of malaria cases in Assam and relation between maximum temperature and minimum temperature with malaria cases. Secondary data from various sources have been collected for this study. Simple linear regression and correlation were applied to analyze correlation between temperature and malaria case by using R version 2.6.2. To draw meaningful conclusion relevant statistical tool like MS-excel was used to perform statistical calculations. Correlation analysis showed that minimum temperature is strongly correlated with positive malaria case in the study area. Special measures are necessary to prevent the transmission of malaria in the study area