Carpesium abrotanoide L. Plant was examined for its biological activities, which included potential for antibacterial, cytotoxic, and antioxidant effects, as well as its phytochemical analyses. According to results of a phytochemical test, Several main and secondary metabolites are lacking in it. Carbohydrates, phenols, saponins, tannins, alkaloids, terpenoids, cardiac glycosides, and phlobatannins are all present in methanolic extract. Oil, glycoside, courmine, and glycoside—all of which were present—were absent from the mixture. It was determined that Escherichia coli, pseudomonas areuginosa, Klebsiella pneumoniae, Acinetobacter baumanni, and Staphylococcus aurus could not grow in the presence of the plant's methanolic extract. However, when tested in methanol against three fungi strains, including Mycospharellacitri, Penicillium, and Trichophyton mentagrophytes, the plant's extract displayed strong antifungal activity against each strain. The potential antibacterial properties of carpesium abrotanoide have been proven, and scientists anticipate that it may one day be employed to treat a variety of diseases and conditions. The experiment using brine shrimp deaths was used to gauge the plant's cytotoxicity (BSLA). It was demonstrated that the herb had cytotoxic effects. The antioxidant capacity of the plant was evaluated using the DPPH method. An extract of the herb Carpesium abrotanoide in methanol was found to have antioxidant properties. Future medical applications will make use of the plant's development results.

Keywords: Phytochemicals, Antimicrobial, Cytotoxic and Antioxidant activity of Carpesium abrotanoide L.