To examine effects of glutathione in regeneration of blood cells after administration of phenobarbital in rats.Investigational drug Glutathione was used as an anti-oxidant drug. Dosage of 30mg/kg body weight of Glutathione was given to GLU group of female Albino Wister rats. Three behavioral studies were examined after administration of glutathione i.e. Open field experiment; particular for kinesis, light & dark experiment; particularly used for depression and home cage experiment; particular for stimulation. These experiments were scored for 5 minutes in 2.5 months. For the determination of the level of regeneration of blood cells, blood sample was collectedby sacrificing animals for hematological estimations by Sysmax (XN-3000 and XN-1000), Cell DYN (Sapphire1-45136AZ, Sapphire2-45197AZ) by Flow Cytometry. Results were carefully compared with control and model groups. Control was untreated group. Model received phenobarbitalas an inducer of dosage 15mg/kg body weight for 15 days and 6mg/kg body weight afterward.Careful observation of results states, locomotion and stimulation, that was gradually reduced after the phenobarbital administration, has steadily but significantly increased when the female Albino Westar were treated with GLU and the depression was markedly disappeared after the treatment in those female rats.On hematology especially on CBC, Glutathione has shown better improvementswhen they were emphasized by comparing with control and model reports.Glutathione has constructive effects on locomotion and stimulation and decrease the level of depression in the body. These activities were depending upon the anti-oxidant concentrations. Glutathione showed better effects on Hematological parameters, especially on CBC.