Otostegia limbata (Benth.) Boiss Family Phytochemical, cytotoxic potential, antifungal, and antibacterial herbal remedies have all been employed previously with the essential understudied ethnomedicinal plant known as Lamiacae. The objective of the current work was to assess with the HPLC analysis, cytotoxic activity, phytochemical analysis, preliminary antibacterial and antifungal activities against oral pathogen of the O. limbata leaf extracts. In various countries since centuries, several diseases are cured related to O. limbata. It diverted our attention to one of its potent species to be unveiled in this research. The focus of this study was to examine systematically its biological activities and seek out its chemical constituents. Qualitative phytochemical analysis removed phenols, glycosides, flavonoids, alkaloids, quinone, carbohydrates, amino acid, terpenes and coumarins are presence while tannins, saponins and sterols are absence. Quantitative phytochemical screening of the O. limbata leaves parts for isolation of active phtometabolites namely alkaloids, sterol, flavonoids, tannins and phenols. The HPLC analysis showed that the samples contained eight identified phenolic compounds, of which Gallic acid, Catechol, Hydroxybenzoic acid, Caffeic acid were most abundant. The antibacterial activity of ethanolic extract displayed the highest inhibition region against Streptococcus mitis that was 35 ± 0.1 (ZOI±SD) and the lowest inhibition region against S .aureus that was 14 ± 0.3(ZOI±SD) in 200mg/ml ± SD.The antifungal activity resulted that ethanol shows the maximum inhibition zone against A.fumigatusthat was 29 ± 0.1 (ZOI±SD) and the minimum inhibition zone against Aspergillus flavus that was 19 ± 0.4 (ZOI±SD) in 200mg/ml ± SD. The ethanolic extract of research plant were exposed to cytotoxic assay at concentrations is 50µg/ml, 100µg/ml and 150µg/ml and their results were calculated that indicates that at 50µg/ml is 60%, 100µg/ml is 70%and 150µg/ml is 80%.The current study suggested that, after the isolation of individual components, O. limbata be investigated for assessing biological activity.The mixture and various combinations of these compounds may indicate a truly potent agent that is novel in its ability to combat a wide range of bacteria and oral pathogens.

Key word: Antibacterial, Antifungal, Cytotoxic, Phytochemicals and HPLC, Otostegia limbata.