Objectives: The primary purpose of this questionnaire study was to assess the knowledge and attitude of Dental health professionals and students concerning dental stem cells.

Materials & Methods: The data collected was via an electronically generated questionnaire which was circulated amongst the house officers and postgraduate residents of all private and public dental universities and hospitals set up in Karachi, Sindh. The questionnaire was used to assess dental professionals' knowledge and attitude toward dental stem cells.

Statistical analysis: Descriptive statistics of socio-demographic variables were presented as mean ± standard deviation or frequency percentages. The outcome variable was assessed in proportion according to knowledge and attitudes. Factor variables were compared using the Chi-square test for categorical variables and the independent t-test for quantitative variables. A p-value of ≤0.05 was considered significant.

Results: Female respondents knew more about stem cells and had more positive attitudes toward stem cell research than male respondents (P≤0.010 and P≤0.035, respectively). There was a significant association between knowledge and attitude about dental stem cells and the age of participants, their area of practice (clinical/academic), and work experience in years (p-value<0.05).

Conclusion: Most participants had adequate knowledge about stem cells and positive attitudes towards both stem cell research scores in female respondents. A significant association of age, type of practice, and work experience with knowledge of dental stem cells and their use in developing non-dental tissues were observed.

Conclusion: A positive attitude towards recommending dental stem cell banking was seen. However, more supervisory oversight and professional action are essential to protect society from unproven stem cell therapies without sufficient clinical trials.

Index Terms: Dental stem cells, dental graduates, knowledge and practices, regenerative dentistry.