Bacteriophage viruses also known as bacterial eaters that infect and eventually kill specific bacteria as their hosts. To date, however very few comprehensive studies of human phage therapy have been performed. Multi drug resistance .Pseudomonas aeruginosa is a pathogen of clinical concern, often associated with cystic fibrosis. Over time it is getting difficult to treat Pseudomonas aeruginosa induced infection using antibiotics and it is necessary to identify new approach. Pseudomonas aeruginosa specific bacteriophage PK was therefore isolated in this study and characterized, in order to check its efficacy as therapeutic agent. By performing spot test phage presence is confirmed and then purified. Optical density of bacterial culture treated with isolated phage was checked, in order to evaluate the reduction in bacterial growth. Phage genomic was extracted and analyzed by gel electrophoresis. pH optimization of phage at different pH (5,6,7,8,9) was noted. Stability of phages under varying temperature (25, 40,60 and 37) was determined.   The isolated phage PK was found to be best stable at 37- degree Celsius. PK phage was also stable under pH 7. PK phage genome is more than 10 kb. Studies on its biological characteristics may provide useful information and knowledge in establishing potential therapeutic agent against Pseudomonas aeruginosa infection.

Key words: Bacteriophage, Drug Resistance, Pseudomonas, cystic fibrosis