Vitex negundo L. is popularly known as Nishinda in Bangladesh regarded as valuable medicinal plant from the ethnobotanical perspectives, which has been using in local areas of Bangladesh from ancient time for curing different types of diseases. The Antibacterial activity of the plant leaves have been evaluated in this study for determination of plant efficacy against some soil bacteria namely Terribacillus sp. 3LF, Bacillus simplex strain TAD155, Bacillus cereus strains 13635K, Bacillus megaterium strains pQ2, Paenibacillus sp. L32 and Paenibacillus sp. BF38. For evaluation of antibacterial activity of Vitex negundo ethanol, chloroform, ethyl acetate and hexane extracts were prepared from powdered leaves. Disc diffusion method was used for study of antibacterial activity of the extracts against the soil bacteria. Among different types of extracts, ethanol extract showed prominent antibacterial activity against nearly all specially the Bacillus cereus strain 13635K considered as the most pathogenic bacteria among the Bacillus genus with inhibition zones of 29mm.

Keywords: Bacillus species, zone of inhibition, minimum inhibitory concentration, antibacterial activity.