Plants are unique source of food, medicinal artifacts, energy and shelter for both human and animal., many useful harvests obtained from plants directly or indirectly validate their importance to the human and other living organisms. Drug discovery, often known as "natural product screening," is the process of evaluating all available medications using pharmacological and phytochemical methods. The current investigation was carried out at Agronomy Laboratory and Microbiology Laboratory, The University of Haripur in order to investigate phytochemical analysis and antimicrobial activity of moringa leaves. Physiochemical and antioxidant evaluation of moringa leaves yielded 6.37% moisture content, 9.66% total ash, 6.41 pH (1% solution), 5.85 pH (10% solution), 0.08% acidity (1% solution), 0.34% acidity (10% solution), 2.59 mg GAE/100g total phenolic contents (TPC), 3.75 mg QE/100g total flavonoids contents and 72.02 DPPH radicals Scavenging activity. Phytochemical screening of water and ethanol extracts showed presence of coumarine, phytosterols, glycosides, tannins, flavonoids and alkaloids in water extracts. Ethanolic extracts showed positive presence of polyphenols, tennins. Antibacterial assay reviled that the highest antibacterial activity of 1mg/ml and 0.5 mg/ml of ethanolic extracts were found to be against E. Coli (17.6, 13.3 and 7.6 mm respectively) followed by S. Aurus (13.6, 8.2 and 7.6 mm), B. Subtilis (10.8, 8.3 and 6.3 mm) and P. aeruginosa (7.7, 5.7, 4.8 mm respectively). Moringa extract also inhibited the development of certain bacteria, demonstrating its anti-microbial potential. This study verifies the presence of important phytochemicals in moringa leaf extracts, leading to the conclusion that moringa leaves can be utilized to cure a variety of ailments.