Background:

Rosa damascene mill is the most commonly used plant because of diverse secondary metabolites. Therefore it was taken in to consideration to determine the vital components of the aqueous extract of flower petals  to carry out  acute toxicity studies of rose water.

Material and method:

The volatile components were studies through HS-GC-MS (headspace-gas chromatography-mass spectrometry).

Results:

 The presence of  total forty-six constituents in sample-1, thirty-eight constituents from sample-2 and sample-3. Among all constituents, ten were common and highlighted as phenyl ethyl alcohol, citronellol, gereniol,nerol, pentadecane, heptadecanol, octadecanol, tetracosane, decane and nonane. The most important compounds identified were citronellol, gereniol, nerol and phenyl ethyl alcohol. Results of  the acute toxicity studies showed that all animals subjected at 15 ml/70kg, 20 ml/70kg and 25ml/70kg adult dose indicated no signs of any irregularities in any test animals during study duration.The brine shrimp lethality bioassay result reflected that all rose water samples tested had LC₅₀ ˃3000µg/ml  showing practically non-toxic.

Conclusion:

From the results, it is evident that rose water from Rosa damascene Mill containing various volatile compounds has strong safety profile, justifying its uses and applications in various phytopharmaceutical formulations.

Keywords: Rose Flower, HS-GC-MS, Fragrance, Volatile Compounds sample, Peak Area, Acute toxicity study, Brine Shrimp Lethality Test.