The main objective of this study is to estimate the concentration of tretinoin in cream tretin and to characterize its standard curve, intra and inter day precision, accuracy, and robustness. Tretinoin has been widely used in anti-acne creams available throughout the world. The methods used in the study were strictly followed under Indian Pharmacopeia. The methodology part includes the method employed for the process of experimentation and revealed a source of information regarding the characteristics and amount of tretinoin in the cream. The maximum absorption wavelength was found by taking 10 µg/ml solution and scanning it using a spectrophotometer (Thermo Fisher Scientific) within a wavelength region of 400-200 nm against ethanol as a blank which is found to be 352 nm. The intraday and interday precision studies indicated good precision of the method. The analyzed samples of tretinoin in three concentrations show a percentage accuracy of 98.35% to 103.81%, which is higher than 100%, indicating the accuracy of the method. The quality of the sample preparation was good to changes in temperature and wavelength. The results obtained showed the values to be under the stipulated values.

Index Terms- Tretinoin, Linearity and range, Precision, LOD, LOQ.