The samples (leaves) were collected from Euphorbia hirta found at their natural habitat of Khanpur Valley in the sub-Himalayan Mountains of Pakistan. Chemical analysis was carried out at the University of Agriculture Peshawar. Significant effects were observed at different seasons and sites upon various biochemical substances. Euphorbia hirta gave higher significant contents for ash (17.95%) at Jabri during summer, crude proteins (15.42%) at Jabri during summer, crude fibers (12.78%) at Mang during winter, crude fats (10.64%) at Jabri during summer, essential oil (1.94%) at Dabola during summer, NFES (59.49%) at Dam during winter, NFEE (198.69%) at Jabri during summer, calcium (199.17mg/100g) at Dam during summer, phosphorus (185.37mg/100g) at Jabri during winter. Euphorbia hirta gave higher values for biochemical during the last week of October.  

Key words: Euphorbia hirta , Himalayan Mountains, Khanpur Valley, biochemical, Jabri