The samples (whole plants) of Ajuga bracteosa were collected from their natural habitat in the Khanpur Valley in the sub-Himalayan Mountains of Pakistan. Chemical analysis was carried out at the University of Agriculture, Peshawar. Significant effects were observed at different seasons and sites on various bio-chemical substances. Ajuga bracteosa revealed higher significant values for ash (34.13%) at Jabri during summer, crude proteins (15.35%) at Dam during summer, crude fats (10.30%) at Jabri during summer, essential oil (2.48%) at Mang during summer, NFES (46.17%) at Dabola during winter, NFEE (186.28%) at Dam during summer, sodium (6.96mg/100g) at Mang during winter, potassium (559.67mg/100g) at Jabri during winter, calcium (254.20mg/100g) at Jabri during summer, magnesium (264.31mg/100g) at Mang during winter; and zinc (6.53mg/100g) at Dam during winter. Ajuga bracteosa gave higher values for bio-chemicals during the last week of October. 

Key Words: Ajuga Bracteosa, habitat, potassium, bio-chemical.