In this study, the experiments were conducted in three agro-ecological zones represented by Swat, Peshawar, and the Horticulture Farm at the University of Haripur, Pakistan, to determine the significant effects of farmyard manure and humic acid on growth, proximate composition, antioxidant leaf pigments, minerals, phytochemical composition and Ascorbic acid of Amaranthus viridis with different levels of FYM (5, 10, and 15 tons/ha) and Humic acid (15, 20, and 30 kg/ha), during the period from March-June 2021. The plots were arranged in randomized complete block design with a split-plot arrangement. Three replications were applied in each district. Results from the single FYM (15 tons/ha) trial indicate significantly affected stem height, root length, leaf area, yield, proximate composition, minerals, antioxidant leaf pigments, vitamin C, TPC, and TFC in all agro-ecological zones. In the case of Humic acid, maximum growth, root length, leaf area, moisture percentage, and dry matter yield proximate composition, minerals, antioxidant leaf pigments, vitamin C, TPC, and TFC were recorded in all regions when compared with FYM 5 ton/ha. In terms of ecological alternation, Overall, Amaranthus viridis performs best in Haripur, then Peshawar and Swat.

Key word: Agro-ecological zones, Amaranthus Viridis, antioxidant leaf pigments, FYM, humic acid, proximate composition.